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American Melanie Oudin produced the upset of the day at Aegon Classic as she ousted 10th-seeded Sorana Cirstea of Romania 3-6, 6-3, 6-2 on Tuesday.
"On grass it's all about holding serve, and I knew if I could do that I'd have a few chances on hers," Oudin said. "In the second and third sets I served well, returned well and played aggressively when I had to."
Also, eleventh seeded Iveta Benesova and 12th seeded Tamira lost to wildcard Tara Moore 6-1, 6-2 and Anne Keothavong 6-2,7-5 respectively.
Another seed Eleni Daniilidou, seeded 16th, lost to France's Stephanie Foretz Gacon 6-4, 6-2.
The only seed to move into the next round was ninth seeded Marina Erakovic.